ramos b cell lysate ramos ra 1 b cells  (ATCC)

Journal: Cancer Science

Article Title: The novel and potent CD40 agonist KHK2840 augments the antitumor efficacy of anti‐ PD ‐1 antibody and paclitaxel

doi: 10.1111/cas.16366

Figure Lengend Snippet: KHK2840 is a novel human CD40 agonist. (A) Representative sensorgrams of hCD40 (left), cynomolgus monkey CD40 (middle left), rat CD40 (middle; right), and mouse CD40 (right) to KHK2840. KHK2840 was captured on the sensor chip immobilized with anti‐human IgG, and then dilutions of recombinant CD40 proteins (0.0625, 0.125, 0.250, 0.500, 1.00 μg/mL) were injected. (B) KHK2840 (red), isotype control (human anti‐DNP IgG2, gray), and positive control (human anti‐DNP IgG1, blue) bind to FcγRIIIa (left, 158F; middle, 158 V) and C1q. Data represent the mean ± SD of three experiments. (C) The in vitro expression of CD80, CD86, and IL‐12p70 in hmoDCs induced by KHK2840 (red) and isotype control (human anti‐DNP IgG2, gray). In IL‐12p70, values below the lower limit of quantification (LLOQ) were replaced with the LLOQ. Data represent the mean ± SD of three wells. The same experiments were repeated in a total of six donors. (D) The in vitro expression of CD86 and CD95 induced in Ramos cells by 0.01 (filled circle), 0.1 (blank circle), 1 (filled square) and 10 μg/mL (blank square) KHK2840 after 0.5, 4, 24, and 48 h of treatment. KHK2840 was washed out at the indicated time points. Cells were analyzed after a total of 48 h of incubation. Data represent the mean ± SD of three wells.

Article Snippet: Human Burkitt's lymphoma Ramos (RA 1) cells (ATCC) were cultured in RPMI1640 supplemented with 10% FBS.

Techniques: Recombinant, Injection, Control, Positive Control, In Vitro, Expressing, Incubation

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: SARS-CoV-2 spike does not interact with the T cell receptor or directly activate T cells.

doi: 10.1073/pnas.2406615121

Figure Lengend Snippet: Fig. 2. SARS-CoV-2 displayed on lentivirus surface does not enable infection of T cells. Viruses pseudotyped with VSVG-mut and SARS-CoV-2 S (wild type and prefusion-stabilized) infect ACE2-expressing Jurkat and CR3022 BCR-expressing Ramos cells and do not infect TCR-expressing Jurkat J76 cells (JM22 and 1G4 TCRs) or off-target VRC01-expressing Ramos cells. Positive controls confirm the functionality of pseudotyped viruses and cell lines: GL9/HLA-A*02:01 virus for JM22 J76 cells and NYESO/HLA-A*02:01 virus for 1G4 J76 cells. Data shown as mean ± SD for n = 3 technical replicates in addition to representative flow cytometry plots. P-values calculated with a one-way ANOVA with Tukey’s multiple comparisons correction. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.

Article Snippet: Jurkat (ATCC TIB- 152), J76 cells, and Ramos cells (ATCC CRL- 1596) were cultured in RPMI- 1640 (ATCC) supplemented with 10% FBS and penicillin- streptomycin.

Techniques: Infection, Expressing, Virus, Flow Cytometry